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KMID : 0364820190550030220
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Korean Journal of Microbiology
2019 Volume.55 No. 3 p.220 ~ p.225
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Comparison between DNA- and cDNA-based gut microbial community analyses using 16S rRNA gene sequences
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Jo Hye-Jun
Hong Ji-Wan
Unno Tatsuya
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Abstract
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Studies based on microbial community analyses have increased in the recent decade since the development of next generation sequencing technology. Associations of gut microbiota with host’s health are one of the major outcomes of microbial ecology filed. The major approach for microbial community analysis includes the sequencing of variable regions of 16S rRNA genes, which does not provide the information of bacterial activities. Here, we conducted RNA-based microbial community analysis and compared results obtained from DNA- and its cDNAbased microbial community analyses. Our results indicated that these two approaches differed in the ratio of Firmicutes and Bacteroidetes, known as an obesity indicator, as well as abundance of some key bacteria in gut metabolisms such as butyrate producers and probiotics strains. Therefore, cDNAbased microbial community may provide different insights regarding roles of gut microbiota compared to the previous studies where DNA-based microbial community analyses were performed.
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KEYWORD
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cDNA, gut microbiota, microbial community analysis, miSeq
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